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JoVE Journal Neuroscience
Magnetic Resonance Spectroscopy of live Drosophila melanogaster using Magic Angle Spinning
 

Magnetic Resonance Spectroscopy of live Drosophila melanogaster using Magic Angle Spinning

Article DOI: 10.3791/1710-v • 07:34 min • April 15th, 2010
April 15th, 2010 •
1,2,3, 2,4, 2,4, 1,2,3
1NMR Surgical Laboratory, Department of Surgery, Massachusetts General Hospital, Harvard Medical School, 2Shriners Burn Institute, 3Department of Radiology, Athinoula A. Martinos Center of Biomedical Imaging, Harvard Medical School, 4Molecular Surgery Laboratory, Department of Surgery, Massachusetts General Hospital, Harvard Medical School

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Summary
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This technique enables the use of high-resolution magic angle spinning proton MR spectroscopy (HRMAS 1H-MRS) for molecular characterization of live Drosophila melanogaster with a conventional 14.1 tesla spectrometer equipped with an HRMAS probe.

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Magnetic Resonance Spectroscopy Live Drosophila Melanogaster Magic Angle Spinning High-Resolution Magic Angle Spinning HRMAS Proton Magnetic Resonance Spectroscopy 1H-MRS Non-destructive Technique Spectral Line-widths High-resolution Spectra Metabolites Cellular Processes In Vivo Studies Fruit Fly Drosophila Melanogaster Genetic Organism Biological Functions Signaling Pathways Genetics And Physiology In Vivo HRMAS 1H-MRS Method 14.1 T MR Spectrometer HRMAS Probe Molecular Characterization Disease Biomarkers Therapeutic Development
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