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Biologie
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Preparation of Bacterial Inoculum for Acinetobacter Biofilm Formation
JoVE Journal
Biologie
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JoVE Journal
Biologie
Preparation of Bacterial Inoculum for
Acinetobacter
Biofilm Formation
Preparation of Bacterial Inoculum for Acinetobacter Biofilm Formation
DOI:
10.3791/200399-v
•
01:38 min
•
August 04, 2023
•
Joo-Sung Kim
2
,
Jihoon Lim
1
Korea Food Research Institute
,
2
Department of Food Biotechnology
,
Korea University of Science and Technology
,
3
Advanced Radiation Technology Institute
,
Korea Atomic Energy Research Institute
Tags
Bacterial Inoculum Preparation For Acinetobacter Biofilm Formation:1. Inoculate A Blood Agar Plate With 2-10 μL Of The Bacterial Strain Using A Sterile Pipette Tip. Streak The Agar Surface
Thinning It Out With Intermittent Flaming. Incubate The Plate At 30°C For 20-24 Hours.2. Select A Single Colony From The Agar Plate And Inoculate 5 ML Of Sterile BHI Broth. Incubate The Inoculated Broth In A Shaking Incubator At 30°C
150 Rpm For 20-24 Hours.3. Transfer 1 ML Of The Culture To A Sterile Microcentrifuge Tube And Centrifuge At 6
000 G For 10 Minutes At Room Temperature. Discard The Supernatant.4. Add 1 ML Of Sterile Phosphate-buffered Saline (PBS) To The Pellet And Resuspend Using A Vortex. Centrifuge At 6
000 G For 10 Minutes At Room Temperature And Discard The Supernatant.5. Resuspend The Resulting Pellet In Sterile
10-fold Diluted BHI Broth Using A Vortex. Ensure The Optical Density Is Around 0.1 At 600 Nm
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