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Centromere-Associated Protein-E Gene Editing Using the CRISPR/Cas9 System

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Transfection, Isolation, and Screening of the CENP-E Knockout HeLa Cells

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Biologie

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JoVE Journal Biologie

Transfection, Isolation, and Screening of the CENP-E Knockout HeLa Cells

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Transfection, Isolation, and Screening of the CENP-E Knockout HeLa Cells

Centromere-Associated Protein-E Gene Editing Using the CRISPR/Cas9 System

DOI:

10.3791/201002-v

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04:10 min

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June 23, 2023

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Meng-Fei Xu², Jie Chen², Yue Xu², Jing-Lian Zhang², Yi Zhou², Jie-Jie He², Shan Wu², Ya-Lan Wei⁴, Zhen-Yu She²

¹Department of Cell Biology and Genetics, The School of Basic Medical Sciences,Fujian Medical University, ²Key Laboratory of Stem Cell Engineering and Regenerative Medicine,Fujian Province University, ³College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics,Fujian Medical University, ⁴Medical Research Center,Fujian Maternity and Child Health Hospital

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Keywords: Transfection HeLa Cells CENP-E Knockout Plasmid Trypsin-EDTA Cell Passage Reduced Serum Medium Transfection Reagent Fluorescence Microscopy Cell Counting Serial Dilution Phenotype-based Screening DNA Extraction PCR Ligation Transformation Sanger Sequencing Wild-type Mutant

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