Extracellular Matrix-derived Foam Fabrication via Lyophilization: A Procedure to Generate Biological Scaffolds from ECM of Decellularized Tissues

Incubate the ECM suspension at 37 degrees Celsius with continuous agitation at 120 rpm until the suspension is warm. Then, use 0.2 molar acetic acid to dilute the sample suspension to the desired concentration. With the 3-milliliter syringe and an 18G needle, fill the desired mold with the ECM suspension.

Foams can be fabricated in a range of geometries depending on the shape of the mold. Cover and freeze the molds at minus 20 or minus 80 degrees Celsius. Then, place the molds into a lyophilizer flask. Connect the flask to the laboratory freeze dryer system and dry the molds for 24 hours or until fully dried.