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如何以文化，记录和刺激神经网络的微电极阵列（多边环境协定）
JoVE Journal
Biologie
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JoVE Journal Biologie
How to Culture, Record and Stimulate Neuronal Networks on Micro-electrode Arrays (MEAs)
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如何以文化，记录和刺激神经网络的微电极阵列（多边环境协定）

DOI:
10.3791/2056-v

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09:27 min

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May 30, 2010

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, ,
1Department of Neurology,Emory University School of Medicine, 2Coulter Department of Biomedical Engineering, Laboratory for Neuroengineering, Georgia Institute of Technology and Emory,University School of Medicine, 3Emory University School of Medicine

Kapitel

  • 00:00Titel
  • 00:51Introduction
  • 01:30Culturing Cortical Neurons on a Micro-Electrode Array (MEA)
  • 05:03Recording From Neuronal Networks on the MEA
  • 07:54Representative Recordings from Cultures On MEAs
  • 08:44Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

该协议提供了必要的成立，关心，录制和电刺激对多边环境协定的文化信息。<em在体外</em>网络提供了一个生理有关的问题，要求在网络和一个更好地理解大脑功能和功能障碍导致的细胞水平的手段。

Tags

CultureRecordStimulateNeuronal NetworksMicro-electrode Arrays (MEAs)NeuroscientistsUnderstandingDiseasesNeuropathological ObservationFluorescent MicroscopyGenetic LabelingIntracellular RecordingDissociated NeuronsSlice PreparationsGrowing Dissociated Neuronal CulturesAdvantagesSimplified ModelNetwork Activity ManipulationElectrical Stimulation SequencesMultiple ElectrodesObservable AreasMonolayer CellsMorphology ChangesImaging TechniquesLong-term Survival In Vitro

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