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Journal / Immunology and Infection / Ex vivo Imaging of T Cells in Murine Lymph Node…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Ex vivo Imaging of T Cells in Murine Lymph Node Slices with Widefield and Confocal Microscopes
 
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Ex vivo Imaging of T Cells in Murine Lymph Node Slices with Widefield and Confocal Microscopes

Article DOI: 10.3791/3054-v • 09:46 min • July 15th, 2011
July 15th, 2011 •
1,2, 1,2, 1,2, 1,2, 1,2, 1,2
1Institut Cochin, Université Paris Descartes, CNRS (UMR 8104), 2Inserm, U1016, Paris, France

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This protocol describes a method to image fluorescent T cells introduced into lymph node slices. The technique permits real-time analyses of T cell migration with traditional widefield fluorescence or confocal microscopes.

Tags

Ex Vivo Imaging T Cells Lymph Node Slices Widefield Microscope Confocal Microscope Migration Of T Cells Chemokines Two-photon Microscopy Quantitative Information Interactions With Other Cells Slice Assay Fluorescently Labeled T Cells Acutely Prepared Lymph Node Slice Real-time Imaging Natural Environment Mechanisms Underlying T Cell Migration
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