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JoVE Journal Bioengineering
Multi-analyte Biochip (MAB) Based on All-solid-state Ion-selective Electrodes (ASSISE) for Physiological Research
 

Multi-analyte Biochip (MAB) Based on All-solid-state Ion-selective Electrodes (ASSISE) for Physiological Research

Article DOI: 10.3791/50020-v • 08:03 min • April 18th, 2013
April 18th, 2013 •
1, 1, 1, 2, 2, 2, 2, 2, 3, 4, 5
1Department of Agricultural and Biological Engineering, Birck-Bindley Physiological Sensing Facility, Purdue University, 2NASA Ames Research Center, 3Department of Chemistry, Pennsylvania State University Hazleton, 4Cooley LLP, 5NASA Life and Physical Sciences, Human Exploration and Operations Mission Directorate, NASA Headquarters

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Summary
Automatic Translation

All-solid-state ion-selective electrodes (ASSISEs) constructed from a conductive polymer (CP) transducer provide several months of functional lifetime in liquid media. Here, we describe the fabrication and calibration process of ASSISEs in a lab-on-a-chip format. The ASSISE is demonstrated to have maintained a near-Nernstian slope profile after prolonged storage in complex biological media.

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Multi-analyte Biochip MAB All-solid-state Ion-selective Electrodes ASSISE Physiological Research Lab-on-a-chip LOC Applications Ion-selective Electrode ISE Microfabricated ASSISE H+ Ca2+ CO32- Ions Noble-metal Electrode Layer Transduction Layer Ion-selective Membrane ISM Layer Conductive Polymer PEDOT Ag/AgCl Transducer Layer Lab-on-a-chip Format Figure 1
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