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Zinc-finger Nuclease Enhanced Gene Targeting in Human Embryonic Stem Cells
 

Zinc-finger Nuclease Enhanced Gene Targeting in Human Embryonic Stem Cells

Article DOI: 10.3791/51764-v • 12:13 min • August 23rd, 2014
August 23rd, 2014 •
1, 1, 1, 1, 1
1Drug Discovery Biology, Monash Institute of Pharmaceutical Sciences, Monash University

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Summary
Automatic Translation

Reporter cell lines offer a means to visualize, track and isolate cells of interest from heterogeneous populations. However, gene-targeting using conventional homologous recombination in human embryonic stem cells is extremely inefficient. Herein, we describe targeting CNS midbrain specific transcription factor PITX3 locus with EGFP using zinc-finger nuclease enhanced homologous recombination.

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Zinc-finger Nuclease Gene Targeting Human Embryonic Stem Cells ESC Differentiation Cell Populations Heterogeneous Undifferentiated ESCs Non-neural Derivatives Neuronal Subtypes In Vitro Applications In Vivo Applications Drug Discovery Cell Replacement Therapy Reporter Cell Lines Visualize Cells Of Interest Track Cells Of Interest Isolate Cells Of Interest Engineered Cell Lines Homologous Recombination Pituitary Homeobox 3 (PITX3) Locus Site-specific Double-strand DNA Breaks PITX3-EGFP-specific DNA Donor Vector PITX3 Reporter Cell Line Parkinson's Disease Modeling
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