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Journal / Medicine / Orthotopic Hind Limb Transplantation in the Mouse
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JoVE Journal Medicine
Orthotopic Hind Limb Transplantation in the Mouse
 

Orthotopic Hind Limb Transplantation in the Mouse

Article DOI: 10.3791/53483-v • 07:15 min • February 12th, 2016
February 12th, 2016 •
*1, *1, *2, 3, 4, 1, 1, 1, 1
1Department of Plastic and Reconstructive Surgery, Vascularized Composite Allotransplantation (VCA) Laboratory, Johns Hopkins University School of Medicine, 2Department of Visceral, Transplant and Thoracic Surgery, Innsbruck Medical University, 3Center for Vascularized Composite Allotransplantation, Department of Plastic and Reconstructive Surgery, Chang Gung Memorial Hospital and School of Medicine, 4Department of General, Visceral and Transplant Surgery, Charite Berlin
* These authors contributed equally

Kapitel

Summary
Automatic Translation

This novel model for orthotopic hind limb transplantation in the mouse, applying a non-suture cuff technique for super-microvascular anastomosis, provides a powerful tool for in vivo mechanistic immunological research related to vascularized composite allotransplantation (VCA).

Tags

Orthotopic Hind Limb Transplantation Mouse Model Non-suture Cuff Technique Super-microvascular Anastomosis Animal Models Reconstructive Transplantation Alloimmune Response Vascularized Composite Allografts Sedation Donor Animal Disinfect Dissecting Microscope Donor Limb Harvest Groin Skin Incision Femoral Artery Vein Nerve Forceps Microscissors Vascular Pedicle
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