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Isolation and Culture of Adult Zebrafish Brain-derived Neurospheres
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Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
Isolation and Culture of Adult Zebrafish Brain-derived Neurospheres

Isolation and Culture of Adult Zebrafish Brain-derived Neurospheres

DOI:
10.3791/53617-v

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11:01 min

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February 29, 2016

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, , , ,
1Yale Cardiovascular Research Center, Internal Medicine,Yale University, 2Department of Medicine,University of California, San Diego, 3APHP Groupe Hospitalier Pitié-Salpètrière,Université Pierre and Marie Curie

Kapitel

  • 00:05Titel
  • 01:02Dissection of Adult Zebrafish Brain
  • 03:25Single Cell Dissociation of Adult Brain
  • 06:07Generation and Passaging of Primary Neurospheres
  • 08:16Results: Analysis of Neurospheres from Adult Zebrafish Brain
  • 09:58Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Here we provide a reproducible method to examine adult neurogenesis using a neurosphere assay derived from the whole brain or from either the telencephalic, tectal or cerebellar regions of the adult zebrafish brain. Additionally, we describe the procedure to manipulate gene expression in zebrafish neurospheres.

Tags

Adult Zebrafish BrainNeurosphere AssayAdult NeurogenesisNeural Stem CellNeural Progenitor CellIn VitroDissectionMicro-dissectionDMEM/F12 Medium

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