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Cancer Research

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JoVE Journal Cancer Research
Labeling of Breast Cancer Patient-derived Xenografts with Traceable Reporters for Tumor Growth and Metastasis Studies
 

Labeling of Breast Cancer Patient-derived Xenografts with Traceable Reporters for Tumor Growth and Metastasis Studies

Article DOI: 10.3791/54944-v • 09:53 min • November 30th, 2016
November 30th, 2016 •
1, 1, 1, 1, 1
1Department of Pathology, School of Medicine, University of Colorado Anschutz Medical Campus

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Summary
Automatic Translation

We describe a method for stable labeling of patient-derived xenografts (PDXs) with lentiviral particles expressing green-fluorescent protein and luciferase reporters. This method allows for tracking the growth of PDXs at the primary site, as well as detecting spontaneous and experimental metastases using in vivo imaging systems.

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Labeling Breast Cancer Patient-derived Xenografts Traceable Reporters Tumor Growth Metastasis Studies Transduction Protocol Reporter Genes PDXs Experimental Models Spontaneous Metastasis Cancer Research Excutaneous Metastasis In Vivo Imaging Histological Analysis Jessica Finlay-Schultz Post-doc Aseptic Technique Scalpel Forceps Wash Medium HBSS HEPES Tissue Culture Plate Tumor Mass
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