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Identification and Characterization of Metastatic Factors by Gene Transfer into the Novel RIP-Tag; RIP-tva Murine Model
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Kapitel
- 00:05Titel
- 00:46Collection and Concentration of Viruses Expressing Metastatic Factor
- 02:26Viral Titer Determination
- 04:37Intracardiac Injection
- 07:24Results: Detection of Metastatic Pancreatic β-cells by Immunostaining
- 08:16Conclusion
We present a protocol to demonstrate a novel somatic gene transfer system utilizing RIP-Tag; RIP-tva mouse model to study the function of genes in metastasis. The avian retroviruses are delivered intracardiacally to ensure gene transfer into pre-malignant, noninvasive lesions of pancreatic β cells in adult mice.
