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Neuroscience

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JoVE Journal Neuroscience
Ablation of a Neuronal Population Using a Two-photon Laser and Its Assessment Using Calcium Imaging and Behavioral Recording in Zebrafish Larvae
 

Ablation of a Neuronal Population Using a Two-photon Laser and Its Assessment Using Calcium Imaging and Behavioral Recording in Zebrafish Larvae

Article DOI: 10.3791/57485-v • 10:29 min • June 2nd, 2018
June 2nd, 2018 •
1, 1
1Division of Molecular and Developmental Biology, National Institute of Genetics, Department of Genetics, SOKENDAI (The Graduate University for Advanced Studies)

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Summary
Automatic Translation

Here, we present a protocol to ablate a genetically labeled subpopulation of neurons by a two-photon laser from Zebrafish larvae.

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Neuronal Population Two-photon Laser Ablation Calcium Imaging Behavioral Recording Zebrafish Larvae Circuit Activity Behavior Experimental Technique Genetic Labeling Spatial Resolution Laser Scanning Microscope Image Acquisition Software Wavelength EGFP Ablation GCaMP Ablation Subjective Lens Fluorescence
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