Journal
/
Genetik
/
Lentiviral Mediated Production of Transgenic Mice: A Simple and Highly Efficient Method for Direct Study of Founders
JoVE Journal
Genetik
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Genetik
Lentiviral Mediated Production of Transgenic Mice: A Simple and Highly Efficient Method for Direct Study of Founders

Lentiviral Mediated Production of Transgenic Mice: A Simple and Highly Efficient Method for Direct Study of Founders

DOI:
10.3791/57609-v

•

10:21 min

•

October 07, 2018

•

, , ,
1UPMC Univ Paris 06, INSERM U1127, CNRS UMR 7225, Institut du Cerveau et de la Moelle épinière, ICM,Sorbonne Universités, 2UPMC Univ Paris 06, INSERM UMRS1166, Institute of Cardiometabolism and Nutrition,Sorbonne Universités

Kapitel

  • 00:04Titel
  • 00:40Collecting Fertilized Eggs from Superovulated Mice
  • 02:49Preparation of Injection Pipette Containing the Lentiviral Vector, Micro-Injection
  • 05:04Transferring Embryos into B6CBAF1/JRj Pseudopregnant Females
  • 08:10Results: Various Tested Constructs
  • 09:28Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Here, we present a protocol to promote transgene integration and production of founder transgenic mice with high efficacy by a simple injection of a lentiviral vector in the perivitelline space of a fertilized oocyte.

Tags

Keywords: LentiviralTransgenic MiceIn Vivo ScreeningGain-of-functionLoss-of-functionDNA Motif MappingOviductFertilized EggsHyaluronidaseCumulus CellsM16 MediumLentiviral VectorMicromanipulatorInjection PipetteHolding PipetteM2 MediumParaffin Oil

Article

Embed

ZUR WIEDERGABELISTE HINZUFÜGEN

Usage Statistics

Verwandte Videos

check_url/de/57609?article_type=v

Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations
check_url/de/57609?article_type=v

Rapid Deletion Production in Fungi via Agrobacterium Mediated Transformation of OSCAR Deletion Constructs
check_url/de/57609?article_type=v

Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage
check_url/de/57609?article_type=v

CRISPR/Cas9-mediated Targeted Integration In Vivo Using a Homology-mediated End Joining-based Strategy
check_url/de/57609?article_type=v

A Protocol for the Production of Integrase-deficient Lentiviral Vectors for CRISPR/Cas9-mediated Gene Knockout in Dividing Cells
check_url/de/57609?article_type=v

Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio
check_url/de/57609?article_type=v

A Fast Silver Staining Protocol Enabling Simple and Efficient Detection of SSR Markers using a Non-denaturing Polyacrylamide Gel
check_url/de/57609?article_type=v

Highly Efficient Gene Disruption of Murine and Human Hematopoietic Progenitor Cells by CRISPR/Cas9
check_url/de/57609?article_type=v

High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture (4C-seq)
check_url/de/57609?article_type=v

CRISPR-mediated Genome Editing of the Human Fungal Pathogen Candida albicans

Read Article