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荧光活化细胞分选分离人皮肤状和网状成纤维细胞

JoVE Journal
Genetik

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JoVE Journal Genetik

Isolation of Papillary and Reticular Fibroblasts from Human Skin by Fluorescence-activated Cell Sorting

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荧光活化细胞分选分离人皮肤状和网状成纤维细胞

DOI:

10.3791/59372-v

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09:14 min

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May 07, 2019

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Ana Korosec*¹, Sophie Frech*¹, Beate M. Lichtenberger

¹Skin, Endothelium Research Division, Department of Dermatology,Medical University of Vienna

Kapitel

00:04Titel
01:00Sectioning of Human Skin Dermis into Papillary and Reticular Layers
02:38Separation and Enzymatic Digestion of Epidermis and Dermis
03:52Preparation of Single Cell Suspension and Erythrocyte Lysis
05:58Culturing of Fibroblasts and Adipogenesis Assay
07:04Results: FAP+CD90-, FAP+CD90+ and FAP-CD90+ Fibroblasts Differ in Dermal Localization, Gene Expression and Functionality
08:44Conclusion

Summary

Automatische Übersetzung

这份手稿描述了一种基于 fac 的从人的皮肤中分离状和网状成纤维细胞的协议。它规避了体外培养, 这是不可避免的与常用的隔离协议通过外植体培养。所产生的成纤维细胞亚群功能不同, 在真皮内显示出不同的基因表达和定位。

Tags

Keyword Extraction: Papillary Fibroblasts Reticular Fibroblasts Human Skin Fluorescence-activated Cell Sorting FACS Dermal Fibroblast Subsets Skin Pathologies Cancer Inflammatory Skin Diseases Full-thickness Dermis Subcutaneous Fat Layer Papillary Dermis Reticular Dermis Enzymatic Digestion Dissociating Enzyme Solution

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