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在生物标志物分析临床试验场单次抽血时,准备外周血单核细胞颗粒和血浆
JoVE Journal
Medizin
This content is Free Access.
JoVE Journal
Medizin
Preparation of Peripheral Blood Mononuclear Cell Pellets and Plasma from a Single Blood Draw at Clinical Trial Sites for Biomarker Analysis
Please note that all translations are automatically generated.
Click here for the English version.
在生物标志物分析临床试验场单次抽血时,准备外周血单核细胞颗粒和血浆
DOI:
10.3791/60776-v
•
07:40 min
•
March 20, 2021
•
Paola Marco-Casanova
,
Natalia Lukashchuk
,
Benedetta Lombardi
,
Veerendra Munugalavadla
,
Melanie M. Frigault
,
Elizabeth A. Harrington
,
J. Carl Barrett
,
Andrew J. Pierce
1
Translational Medicine, R&D Oncology
,
AstraZeneca
,
2
Acerta Pharma, AstraZeneca Group
,
3
Translational Medicine, R&D Oncology
,
AstraZeneca
Kapitel
00:05
Introduction
01:08
Whole Blood Irradiation for DNA Damage Response (DDR) Biomarker Expression Analysis
03:35
PBMC and Plasma Freezing
04:45
Western Blot Analysis
05:43
Results: Representative DNA Damage Assessment
07:10
Conclusion
Summary
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Automatische Übersetzung
本协议详细介绍了可用于转化生物标志物分析的临床试验现场高质量PBMC和血浆生物采样剂的临床可实施制备。
Tags
PBMC
Plasma
Blood Draw
Biomarker Analysis
Clinical Trial
Pre-analytical Variability
Pharmacokinetics
Pharmacodynamics
DNA Damage Response
Radiation
Centrifugation
Cell Separation
Buffy Coat
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