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Immunology and Infection
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JoVE Journal Immunology and Infection
Optimization of the Cuff Technique for Murine Heart Transplantation
 

Optimization of the Cuff Technique for Murine Heart Transplantation

Article DOI: 10.3791/61103-v • 14:01 min • June 26th, 2020
June 26th, 2020 •
*1,2, *1,2, *3,4,5, 6, 6, 7, 1,2, 1,2,6, 1,2,8
1Organ Transplantation Institute, School of Medicine, Xiamen University, 2Fujian Key Laboratory of Organ and Tissue Regeneration, 3Department of Kidney Transplantation, Center of Organ Transplantation, The Second Xiangya Hospital of Central South University, 4Clinical Research Center for Organ Transplantation in Hunan Province, 5Clinical Immunology Center, Central South University, 6School of Medicine, Xiamen University, 7Xiamen Medical College, 8Medical College, Guangxi University
* These authors contributed equally

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Summary
Automatic Translation

We introduce an inner tube approach to the cuff technique for mouse cervical heterotopic heart transplantation to help evert the vessel over the cuff. We found that cooperation between two experienced surgeons markedly shortens the operation time.

Tags

Cuff Technique Murine Heart Transplantation Optimization Surgical Technique Surgical Efficiency Insertion Tube Technique Skilled Surgeon Success Rate Thermal Surgeon Operation Module Operation Time Atraumatic Mechanical Clippers Hair Removal Iodine Antiseptic Ethanol Supine Position Transverse Incision External Jugular Vein
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