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Cancer Research

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JoVE Journal Cancer Research
Immunofluorescence Imaging of DNA Damage and Repair Foci in Human Colon Cancer Cells
 

Immunofluorescence Imaging of DNA Damage and Repair Foci in Human Colon Cancer Cells

Article DOI: 10.3791/61399-v • 05:18 min • June 9th, 2020
June 9th, 2020 •
1, 1, 1, 1, 1
1Nuclear Facilities Operations Department, National Centre for Nuclear Research, Sołtana 7, 05-400 Otwock, Poland

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Summary
Automatic Translation

Radiation-induced DNA damage response activated by neutron mixed-beam used in boron neutron capture therapy (BNCT) has not been fully established. This protocol provides a step-by-step procedure to detect radiation-induced foci (RIF) of repair proteins by immunofluorescence staining in human colon cancer cell lines after irradiation with the neutron-mixed beam.

Tags

Immunofluorescence Imaging DNA Damage Repair Foci Human Colon Cancer Cells Radiation-induced Foci Immunofluorescence Staining Neutron-mixed Beam Repair Pathway Proteins DNA Damaging-agents Ionizing Radiation Boron Neutron Capture Therapy BNCT Radiation-induced DNA Damage Response High-LET Beams Protons Proton Beam Therapy Carbon Ions Hadron Therapy Medium Removal PBS Wash Ethanol Fixation Cell Permeabilization Triton X-100 BSA Blockage Primary Antibody Staining
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