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Journal / Developmental Biology / 早期果蝇胚胎内免疫组织化学和RNA原位分布的方案…
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Developmental Biology
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JoVE Journal Developmental Biology
早期果蝇胚胎内免疫组织化学和RNA原位分布的方案
 
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早期果蝇胚胎内免疫组织化学和RNA原位分布的方案

Article DOI: 10.3791/61776-v • 14:34 min • May 6th, 2022
May 6th, 2022 •
*1, *1, *2,3, 1, 1, 1, 1, 1, 1, 1,4, 1, 5, 6, 7, 3, 1
1National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China/CGDB, Shaanxi Normal University College of Life Sciences, 2Shaanxi Key Laboratory of Ischemic Cardiovascular Disease, Shaanxi Key Laboratory of Brain disorders, Institute of Basic & Translational Medicine, Xi'an Medical University, 3Ohio State University College of Medicine, 4Hematology-Oncology Division, Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, 5University of Pennsylvania ULAR, 6University of Maryland School of Medicine, 7Columbia University Medical Center
* These authors contributed equally

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在这里，我们描述了果 蝇 胚胎蛋白和RNA的检测和定位方案，从收集到预包埋和包埋，免疫染色和mRNA 原位 杂交。

Tags

发育生物学，第183期， 果蝇 胚胎，石蜡切片，包衣载玻片，包埋， 原位 杂交，DmFKBP12 /Calstabin
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