Journal
/
Medizin
/
A Model of Experimental Steatosis In Vitro: Hepatocyte Cell Culture in Lipid Overload-Conditioned Medium
JoVE Journal
Medizin
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Medizin
A Model of Experimental Steatosis In Vitro: Hepatocyte Cell Culture in Lipid Overload-Conditioned Medium

A Model of Experimental Steatosis In Vitro: Hepatocyte Cell Culture in Lipid Overload-Conditioned Medium

DOI:
10.3791/62543-v

•

08:35 min

•

May 18, 2021

•

,
1Laboratorio de Hígado, Páncreas y Motilidad, Unidad de Medicina Experimental, Facultad de Medicina-UNAM,Hospital General de México “Dr. Eduardo Liceaga”

Kapitel

  • 00:05Introduction
  • 00:55Standard and Conditioned Medium Preparation
  • 03:11Steatogenic Culture
  • 03:48Viability and Mortality Assessment
  • 04:44Lipid Staining with Oil-Red O
  • 05:46Morphometric Assessment of Lipid Contents
  • 06:29Results: Hepatocyte Culture in Steatogenic Conditions
  • 07:44Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

This protocol is intended to be a tool to study steatosis and the molecular, biochemical, cellular changes produced by the over exposure of hepatocytes to lipids in vitro.

Tags

Liver SteatosisIn VitroHepatocyte Cell CultureLipid OverloadPalmitateOleateSteatogenic MediumMild SteatosisSevere SteatosisRPMI 1640Fatty AcidsLipid-free BSAReproducibilityDiagnostic MarkersTherapeutic Targets

Article

Embed

ZUR WIEDERGABELISTE HINZUFÜGEN

Usage Statistics

Verwandte Videos

Multi-photon Imaging of Tumor Cell Invasion in an Orthotopic Mouse Model of Oral Squamous Cell Carcinoma

Stem Cell Transplantation in an in vitro Simulated Ischemia/Reperfusion Model

Assessing Teratogenic Changes in a Zebrafish Model of Fetal Alcohol Exposure

In vitro Mesothelial Clearance Assay that Models the Early Steps of Ovarian Cancer Metastasis

Excitotoxic Stimulation of Brain Microslices as an In vitro Model of Stroke

Neural Stem Cell Transplantation in Experimental Contusive Model of Spinal Cord Injury

Optimized Analysis of In Vivo and In Vitro Hepatic Steatosis

Using In Vitro Live-cell Imaging to Explore Chemotherapeutics Delivered by Lipid-based Nanoparticles

A Cell Culture Model of Resistance Arteries

Dissection and Explant Culture of Murine Allantois for the In Vitro Analysis of Allantoic Attachment

Read Article