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Journal
/
Krebsforschung
/
Pancreatic Cancer-Derived Tumor Organoids and Fibroblasts from Fresh Tissue
/
Author Spotlight: Establishment of Pancreatic Cancer-Derived Tumor Organoids and Fibroblasts From Fresh Tissue
JoVE Journal
Krebsforschung
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JoVE Journal
Krebsforschung
Establishment of Pancreatic Cancer-Derived Tumor Organoids and Fibroblasts From Fresh Tissue
Author Spotlight: Establishment of Pancreatic Cancer-Derived Tumor Organoids and Fibroblasts From Fresh Tissue
Pancreatic Cancer-Derived Tumor Organoids and Fibroblasts from Fresh Tissue
DOI:
10.3791/65229-v
•
02:11 min
•
May 26, 2023
•
Jesús Frutos Díaz-Alejo*
1,2,3,4
,
Simon April-Monn*
5
,
Marina Cihova
,
Verona Buocikova
,
Jorge Villalón López
3
,
Maria Urbanova
,
Carmen G. Lechuga
,
Miroslav Tomas
8
,
Peter Dubovan
8
,
Bárbara Luna Sánchez
,
Sonia Camaño Páez
,
Alfonso Sanjuanbenito
9
,
Eduardo Lobo
,
Estefanía Romio de la Heras
,
Carmen Guerra
7
,
Carolina de la Pinta
,
Emma Barreto Melian
2
,
Mercedes Rodríguez Garrote
2
,
Alfredo Carrato
2,4
,
Laura Ruiz-Cañas
12,13
,
Bruno Sainz, Jr.
3,12,13
,
Ana Torres
,
Bozena Smolkova
,
Julie Earl
2,3
1
Molecular Epidemiology and Predictive Tumor Markers Group, Area 3
,
Ramón y Cajal Health Research Institute (IRYCIS)
,
2
The Biomedical Research Network in Cancer (CIBERONC)
,
3
Biobank and Biomodels Platform (PT20/0045), ISCIII research and development platforms in biomedicine and health sciences, BioBank Hospital Ramón y Cajal-IRYCIS, Spanish National Biobanks Network (ISCIII Biobank Register No. B.0000678)
,
Ramón y Cajal Health Research Institute (IRYCIS)
,
4
Faculty of Medicine
,
University of Alcalá de Henares
,
5
Institute of Tissue Medicine and Pathology
,
University of Bern
,
6
Department of Molecular Oncology, Cancer Research Institute
,
Biomedical Research Center of the Slovak Academy of Sciences
,
7
Experimental Oncology, Molecular Oncology Program
,
Centro Nacional de Investigaciones Oncológicas (CNIO)
,
8
Department of Surgical Oncology, National Cancer Institute
,
Slovak Medical University
,
9
Pancreatic and Biliopancreatic Surgery Unit
,
Hospital Universitario Ramón y Cajal
,
10
Department of Pathology
,
Hospital Universitario Ramón y Cajal
,
11
Department of Radiation Oncology
,
Hospital Universitario Ramón y Cajal
,
12
Department of Cancer
,
Instituto de Investigaciones Biomédicas “Alberto Sols” (IIBM)
,
13
Cancer Stem Cell and Fibroinflammatory Group, Chronic Diseases and Cancer, Area 3
,
IRYCIS
Tags
Tumor Organoids
Pancreatic Cancer
Tumor Microenvironment
Personalized Medicine
Translational Research
In Vitro Models
In Vivo Models
Spatial Transcriptomics
Molecular Subgroups
Standardized Protocol
Cell Culture Techniques
Growth Factor Cocktails
Extracellular Matrix
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Author Spotlight: Establishment of Pancreatic Cancer-Derived Tumor Organoids and Fibroblasts From Fresh Tissue
Fresh Primary Tumor Tissue Processing to Establish Tumor Organoids and Primary Fibroblasts
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