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Journal
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Biotechnik
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3D Co-Culture Assay: Vascularized Osteogenic Niches for Cancer Drug Screening
/
Author Spotlight: Simple Establishment of a Vascularized Osteogenic Bone Marrow Niche Using Pre-Cast Poly(Ethylene Glycol) (PEG) Hydrogels in an Imaging Microplate
JoVE Journal
Biotechnik
This content is Free Access.
JoVE Journal
Biotechnik
Simple Establishment of a Vascularized Osteogenic Bone Marrow Niche Using Pre-Cast Poly(ethylene Glycol) (PEG) Hydrogels in an Imaging Microplate
Author Spotlight: Simple Establishment of a Vascularized Osteogenic Bone Marrow Niche Using Pre-Cast Poly(Ethylene Glycol) (PEG) Hydrogels in an Imaging Microplate
3D Co-Culture Assay: Vascularized Osteogenic Niches for Cancer Drug Screening
DOI:
10.3791/65413-v
•
01:15 min
•
May 19, 2023
•
Lisa A. Krattiger
,
Maria Mitsi
,
Benjamin R. Simona
,
Martin Ehrbar
1
Department of Obstetrics
,
University Hospital Zurich, University of Zurich
,
2
Ectica Technologies AG
Tags
Vascularized Osteogenic Bone Marrow Niche
PEG Hydrogels
3D Co-culture
HBM-MSCs
HUVECs
In Vitro Model
Synthetic Matrix
Microscopy
Bone And Bone Marrow
Drug Screening
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Verwandte Videos
Author Spotlight: Simple Establishment of a Vascularized Osteogenic Bone Marrow Niche Using Pre-Cast Poly(Ethylene Glycol) (PEG) Hydrogels in an Imaging Microplate
Establishing the 3D Stromal Monoculture
Establishing the 3D Stromal-Endothelial Cell Co-Culture
Quantifying the Endothelial Cell Network Formation After the 3D Stromal-Endothelial Cell Co-Culture
Assessing the Osteogenic Differentiation of 3D Stromal-Endothelial Cells by Monitoring the Alkaline Phosphatase Activity
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