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FACS Analysis of GFP and mCherry Reporter Cells to Evaluate NHEJ & HR Efficiency
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Author Spotlight: Decoding DNA Repair by Extrachromosomal NHEJ Assay and HR Assays
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Biologie
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JoVE Journal Biologie
Analysis of Nonhomologous End Joining and Homologous Recombination Efficiency in HEK-293T Cells Using GFP-Based Reporter Systems

Author Spotlight: Decoding DNA Repair by Extrachromosomal NHEJ Assay and HR Assays

FACS Analysis of GFP and mCherry Reporter Cells to Evaluate NHEJ & HR Efficiency

DOI:
10.3791/66501-v

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02:29 min

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February 02, 2024

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, , , , ,
1Laboratory of Protein Structure and Function, Institute of Medicine and Pharmacy,Qiqihar Medical University

Tags

NHEJHomologous RecombinationDNA Double-strand BreaksGFP Reporter SystemHEK-293T CellsFlow CytometryExtrachromosomal AssayI-SceI Endonuclease

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