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Preparation of Stria Vascularis Single-Nucleus Suspension (Video) | JoVE

Published: April 21, 2023

doi:

Dillon Strepay, Rafal Olszewski, Ian Taukulis, J. Dixon Johns, Shoujun Gu, Michael Hoa

¹Auditory Development and Restoration Program, National Institute on Deafness and Other Communication Disorders,National Institutes of Health

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Stria Vascularis Single-nucleus Suspension Homogenize Dounce Homogenizer Lyse Filter Centrifuge Supernatant Resuspend Nuclei Wash Resuspension Buffer Trypan Blue Single Nuclei Captures Cell Density Cell Suspension

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Preparation of Stria Vascularis Single-Nucleus Suspension. J. Vis. Exp. (Pending Publication), e200195, doi:10.3791/200195 (2023).

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