Encyclopedia Of Experiments

Breast Cancer

This collection features biomedical research methods employed in research laboratories to advance breast cancer prevention, detection, and treatment.

Encyclopedia Of Experiments

Breast Cancer

This collection features biomedical research methods employed in research laboratories to advance breast cancer prevention, detection, and treatment.

Sections

Procedures and techniques

In vitro studies

Procedures and techniques

In vitro studies

Procedures and techniques

Orthotopic Injection into the Mammary Fat Pad

Establishing Breast Cancer in Mice

Lymphedema Ultrasonography

A Technique to Measure the Change in Thickness of an Affected Tissue

Orthotopic Injection

Implanting Tissue Specific Cancer Cells into an Adult Mouse

Radical Mastectomy

Surgical Removal of the Entire Mammary Gland from a Mouse to Study Cancer Progression

Intraductal Injection

Delivering Injection Mix into the Ducts of the Mouse Mammary Gland

zPDX-Analysis of Invasiveness

Investigating Invasive Behavior of Metastatic Cancer Cells in Zebrafish Embryo Xenografts

Portal Vein Injection

A Method to Study Cancer Metastasis to the Liver

India Ink Inflation

A Staining Method to Visualize Tumor Nodules

Spatial Measurement of Tumor Interstitial Fluid Pressure

A Method to Measure the Interstitial Fluid Pressure

Rabbit Intraductal Injection

Localized Delivery of Solution of Interest into the Rabbit Mammary Gland

Mechanical Dissociation

A Method to Obtain Viable Cells from a Tissue

Sample Preparation for Metabolomics

A Method to Prepare Cell Samples for Metabolite Profiling

Tail Vein Injection

A Method to Administer Cancer Cells for Metastatic Studies in a Mouse Model

Decellularizing Mammary Fat Pads

Deriving Extracellular Matrix Hydrogels from Mammary Fat Pads

In vitro studies

Generation of Primary Mammospheres

A Technique to Determine Cell Potency

Transduction to Label PDX Tumor Cells

Introducing Lentivirus Expressing Fluorescent Marker into the Tumor Cell In Vitro

Co-culturing BC Cells

Culturing Breast Cancer Cells with Bone Fragments to Study Cancer Cell Proliferation

3D Co-culture with Direct Interaction

Co-culturing Cancer Cells with Monocytes to Study Their Interaction

Organ Conditioned Medium

Generating Medium from Harvested Organ Cells for Metastatic Cancer Studies

3D Culture Basement Membrane Assay

Culturing Cancer Cells on 3D Basement Membrane Protein Matrix to Study Cell Invasion

Soft Agar Colony Formation Assay

A Method to Test Effects of Novel Compounds on Cancer Cell Proliferation