Assessing the Osteogenic Differentiation of 3D Stromal-Endothelial Cells by Monitoring the Alkaline Phosphatase Activity

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Assessing the Osteogenic Differentiation of 3D Stromal-Endothelial Cells by Monitoring the Alkaline Phosphatase Activity (Video) | JoVE

Published: May 19, 2023

doi:

10.3791/200259

Lisa A. Krattiger, Maria Mitsi, Benjamin R. Simona, Martin Ehrbar

¹Department of Obstetrics,University Hospital Zurich, University of Zurich, ²Ectica Technologies AG

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Keywords: 3D Stromal-endothelial Cells Osteogenic Differentiation Alkaline Phosphatase Activity HBM-MSCs GFP-HUVECs BCIP/NBT Substrate Cell Lysates ALP Activity Quantification DNA Quantification Mesenchymal Stem Cells

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Assessing the Osteogenic Differentiation of 3D Stromal-Endothelial Cells by Monitoring the Alkaline Phosphatase Activity. J. Vis. Exp. (Pending Publication), e200259, doi:10.3791/200259 (2023).

Assessing the Osteogenic Differentiation of 3D Stromal-Endothelial Cells by Monitoring the Alkaline Phosphatase Activity (Video) | JoVE

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