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Nitrotetrazolium Blue Chloride (NBT) Assay for Evaluating ROS Production (Video) | JoVE

Published: February 09, 2024

doi:

Isabelle Souza Luz, Raquel Takaya, Daiane Gonzaga Ribeiro, Natanael Sales Silva, Lucas Fontes, Mariana S. Castro, Wagner Fontes

¹Laboratory of Protein Chemistry and Biochemistry, Department of Cell Biology,University of Brasilia, ²Institute of Physics,University of Brasilia

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Keywords: NBT Assay ROS Production Polymorphonuclear Neutrophils DMSO HBSS PMA FMLP NBT Slide Test NBT Spectrophotometry Assay Formazan Deposits SDS Sonication Absorbance Measurement

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Nitrotetrazolium Blue Chloride (NBT) Assay for Evaluating ROS Production. J. Vis. Exp. (Pending Publication), e200484, doi:10.3791/200484 (2024).

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