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Culturing H9-Embryonic Stem Cells for Generation of Human Neural Retina

Published: December 22, 2023

doi:

Wen Li*^1,2, Hongyu Li*³, Hao Yan, Lixiong Gao, Xiaoling Wang, Lingping Zhao, Yuhan Yan², Zi Ye, Jiafei Xi, Wen Yue, Zhaohui Li²

¹Medical School of Chinese PLA, ²Senior Department of Ophthalmology,The Third Medical Center of Chinese PLA General Hospital, ³Stem Cell and Regenerative Medicine Lab,Beijing Institute of Radiation Medicine

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Keywords: H9-Embryonic Stem Cells Neural Retina Extracellular Matrix Maintenance Medium Y-27632 Centrifugation Cell Culture Cell Density EDTA PBS Incubation Carbon Dioxide

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Culturing H9-Embryonic Stem Cells for Generation of Human Neural Retina. J. Vis. Exp. (Pending Publication), e201241, doi:10.3791/201241 (2023).

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