Cell Lysis of Transfected HEK293 Cells and Protein Sample Preparation for Immunoprecipitation

doi:10.3791/201503

JoVE Journal > Biology

Biología

Cell Lysis of Transfected HEK293 Cells and Protein Sample Preparation for Immunoprecipitation

Published: January 05, 2024

doi:

10.3791/201503

Hiroko Shinjo, Gaku Nagano, Shogo Ishii, Natsumi Himeno, Yutaro Yamamoto, Junji Sagawa, Ryuta Baba, Gentaro Egusa, Noboru Hattori, Haruya Ohno

¹Department of Molecular and Internal Medicine, Graduate School of Biomedical & Health Sciences,Hiroshima University

Tags

Cell Lysis HEK293 Cells Protein Sample Preparation Immunoprecipitation Transfection Protein Lysis Buffer Protease Inhibitors Cell Scraper Centrifugation Protein Concentration Protein Quantification

Play Video

PDF

DOI

Citar este artículo

Cell Lysis of Transfected HEK293 Cells and Protein Sample Preparation for Immunoprecipitation. J. Vis. Exp. (Pending Publication), e201503, doi:10.3791/201503 (2024).

Cell Lysis of Transfected HEK293 Cells and Protein Sample Preparation for Immunoprecipitation

Tags

Play Video

Citar este artículo

View Video

✖

To prove you're not a robot, please enter the text in the image below