Ultra-Fast Amplicon-Based Next-Generation Sequencing in Non-Squamous Non-Small Cell Lung Cancer

Christophe Bontoux; Virginie Lespinet-Fabre; Olivier Bordone; Virginie Tanga; Maryline Allegra; Myriam Salah; Salom&#233; Lalv&#233;e; Samantha Goffinet; Jonathan Benzaquen; Charles-Hugo Marquette; Marius Ili&#233;; V&#233;ronique Hofman; Paul Hofman

doi:10.3791/65190

Assessing Genomic Alteration in Non-Squamous Non-Small Cell Lung Cancer Patients

September 08, 2023

Advancements in Molecular Biomarker Testing for Non-Squamous Non-Small Cell Lung Cancer (Video) | JoVE

02:14 min

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Preparation of FFPE DNA and RNA Samples for Sequencing Using an Automated Purification Instrument (API) (Video) | JoVE

02:34 min

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Amplicon-Based Next-Generation Sequencing of Non-Squamous Non-Small Cell Lung Cancer (Video) | JoVE

01:13 min

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Sequencing Analysis of Non-Squamous Non-Small Cell Lung Cancer Using Integrated Genomic Viewer Software (Video) | JoVE

02:11 min

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Christophe Bontoux^2,3,4,5, Virginie Lespinet-Fabre^2,3,4, Olivier Bordone^2,3,4, Virginie Tanga^2,3,4, Maryline Allegra^2,3,4, Myriam Salah^2,3,4, Salomé Lalvée^2,3,4, Samantha Goffinet^2,3,4, Jonathan Benzaquen^4,5,6, Charles-Hugo Marquette^4,5,6, Marius Ilié^2,3,4,5, Véronique Hofman^2,3,4,5, Paul Hofman^2,3,4,5

¹Laboratory of Clinical and Experimental Pathology, Centre Hospitalier Universitaire de Nice,Université Côte d’Azur, CHU de Nice, ²Hospital-Integrated Biobank (BB-0033-00025),Université Côte d’Azur, Hôpital Pasteur, CHU de Nice, ³Institut Hospitalo-Universitaire (IHU), RespirERA,Université Côte d’Azur, Hôpital Pasteur, CHU de Nice, ⁴FHU OncoAge,Université Côte d’Azur, ⁵Team 4, Institute of Research on Cancer and Aging (IRCAN), CNRS INSERM, Centre Antoine-Lacassagne,Université Côte d’Azur, ⁶Department of Pulmonary Medicine and Thoracic Oncology, Centre Hospitalier Universitaire de Nice,Université Côte d’Azur

Summary

The increase of molecular biomarkers to be tested for non-squamous non-small cell lung cancer (NS-NSCLC) care management has prompted the development of fast and reliable molecular detection methods. We describe a workflow for genomic alteration assessment for NS-NSCLC patients using an ultra-fast-next generation sequencing (NGS) approach.