Optogenetic Signaling Activation in Zebrafish Embryos

Allison J. Saul; Catherine E. Rogers; Marcial Garmendia-Cedillos; Thomas Pohida; Katherine  W. Rogers

doi:10.3791/65733

Investigating Development with Optogenetic Signaling Manipulation in Zebrafish

October 27, 2023

Manipulating Signaling in Zebrafish Embryos to Decode Cell Fate Decisions (Video) | JoVE

01:07 min

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Injecting bOpto-BMP/Nodal Constructs into Zebrafish Embryos (Video) | JoVE

01:56 min

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Light Exposure Experiment to Activate BMP or Nodal Signaling in Zebrafish (Video) | JoVE

02:25 min

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Evaluation of bOpto-BMP/Nodal Expressing Embryos After the Light Exposure Experiment (Video) | JoVE

01:48 min

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Allison J. Saul*¹, Catherine E. Rogers*¹, Marcial Garmendia-Cedillos, Thomas Pohida, Katherine W. Rogers

¹Division of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development,NIH, ²Instrumentation Development and Engineering Application Solutions, National Institute of Biomedical Imaging and Bioengineering,NIH

Summary

Optogenetic manipulation of signaling pathways can be a powerful strategy to investigate how signaling is decoded in development, regeneration, homeostasis, and disease. This protocol provides practical guidelines for using light-oxygen-voltage sensing domain-based Nodal and bone morphogenic protein (BMP) signaling activators in the early zebrafish embryo.