Generating Retinal Injury Models in <em>Xenopus</em> Tadpoles

Karine Parain; Alicia Donval; Albert Chesneau; Jing Xian Lun; Caroline Borday; Muriel Perron

doi:10.3791/65771

Generating Retinal Degeneration to Investigate Cellular Repair Strategies in Xenopus

October 13, 2023

Generating Retinal Injury Models in Xenopus Tadpoles

02:21 min

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Mechanical Retinal Puncture in Xenopus Tadpoles to Study Retinal Regeneration Mechanisms

01:27 min

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Conditional Rod Cell Ablation in Xenopus Tadpoles Using the NTR-MTZ System

02:05 min

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Rod Cell Degeneration in Xenopus Tadpoles by CRISPR/Cas9-Mediated Rhodopsin Knockout

01:38 min

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Retinal Cell Degeneration in Xenopus Tadpoles by Cytotoxic Intraocular CoCl₂ Injections

02:12 min

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Karine Parain*¹, Alicia Donval*¹, Albert Chesneau*¹, Jing Xian Lun, Caroline Borday, Muriel Perron

¹Paris-Saclay Institute of Neuroscience, CNRS,Université Paris-Saclay

Summary

We have developed several protocols to induce retinal damage or retinal degeneration in Xenopus laevis tadpoles. These models offer the possibility of studying retinal regeneration mechanisms.