Oxitec Ltd 1 article published in JoVE Environment Mass Production of Genetically Modified Aedes aegypti for Field Releases in Brazil Danilo O. Carvalho1,2, Derric Nimmo1, Neil Naish1, Andrew R. McKemey1, Pam Gray1, André B. B. Wilke3, Mauro T. Marrelli3, Jair F. Virginio4, Luke Alphey1,5, Margareth L. Capurro2,6 1Oxitec Ltd, 2Departamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, 3Departamento de Epidemiologia, Universidade de São Paulo, 4Moscamed Brasil, 5Deptartment of Zoology, University of Oxford, 6Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular (INCT-EM) To achieve population suppression of Aedes aegypti using the RIDL® (Release of Insects carrying a Dominant Lethal) system, large numbers of male mosquitoes need to be released. This requires the use of mass rearing techniques and technology to provide reliable systems to obtain the maximum number of high quality male mosquitoes.