All Protocols and Video Articles for scientific research | JoVE

Showing 85 - 96 of 164 videos for "INDEL"

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Journal (Medicine)

Targeting Gray Rami Communicantes in Selective Chemical Lumbar Sympathectomy

Wen-Hui Wang, Long Zhang³, Guo-Xiang Dong, Jun Zhao, Xuan Li³

¹Department of Dermatology,Peking University Third Hospital, ²Department of Interventional Radiology and Vascular Surgery,Peking University Third Hospital, ³Wound Healing Center,Peking University Third Hospital

Journal (Genetics)

CRISPR-mediated Genome Editing of the Human Fungal Pathogen Candida albicans

Ben A. Evans*¹, Ethan S. Pickerill*¹, Valmik K. Vyas, Douglas A. Bernstein

¹Department of Biology,Ball State University, ²Whitehead Institute for Biomedical Research

Journal (Cancer Research)

Investigation of Genetic Dependencies Using CRISPR-Cas9-based Competition Assays

Anagha Deshpande, Bo Rui Chen, Luyi Zhao, Kelsey Saddoris, Mayuri Kerr, Nan Zhu, Prashant Mali, Aniruddha J. Deshpande

¹Tumor Initiation and Maintenance Program,Sanford Burnham Prebys Medical Discovery Institute, ²Blood Research Institute,Blood Center of Wisconsin, ³Department of Bioengineering,University of California, San Diego

Journal (Immunology and Infection)

Generation of Knock-out Primary and Expanded Human NK Cells Using Cas9 Ribonucleoproteins

Meisam Naeimi Kararoudi, Hamid Dolatshad, Prashant Trikha, Syed-Rehan A. Hussain, Ezgi Elmas, Jennifer A. Foltz, Jena E. Moseman, Aarohi Thakkar, Robin J. Nakkula, Margaret Lamb, Nitin Chakravarti, K. John McLaughlin, Dean A. Lee

¹Center for Childhood Cancer and Blood Disease,Nationwide Children's Hospital, ²Nuffield Division of Clinical Laboratory Sciences, Radcliffe Department of Medicine,University of Oxford, ³Center for Clinical and Translational Research,Nationwide Children's Hospital Research Institute

Journal (Immunology and Infection)

Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing

Na Tang², Yaoyao Zhang, Miriam Pedrera, Pengxiang Chang, Susan Baigent, Katy Moffat, Zhiqiang Shen, Venugopal Nair, Yongxiu Yao

¹The Pirbright Institute & UK-China Centre of Excellence for Research on Avian Diseases, ²Binzhou Animal Science and Veterinary Medicine Academy & UK-China Centre of Excellence for Research on Avian Diseases

Journal (Genetics)

Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9

Amanda Haupt, Tanya Grancharova, Joy Arakaki, Margaret A. Fuqua, Brock Roberts, Ruwanthi N. Gunawardane

¹Allen Institute for Cell Science

Journal (Biology)

Visualizing Genetic Variants, Short Targets, and Point Mutations in the Morphological Tissue Context with an RNA In Situ Hybridization Assay

Courtney M. Anderson, Annelies Laeremans, Xiao-Ming Mindy Wang, Xingyong Wu, Bingqing Zhang, Emerald Doolittle, Jeffrey Kim, Na Li, Helly Xiao Yan Pimentel, Emily Park, Xiao-Jun Ma

¹Advanced Cell Diagnostics, Inc

Journal (Developmental Biology)

Whole Mount Immunofluorescence and Follicle Quantification of Cultured Mouse Ovaries

Vera D. Rinaldi, Jordana C. Bloom, John C. Schimenti²

¹Department of Biomedical Sciences,Cornell University, ²Department of Molecular Biology and Genetics,Cornell University

Journal (Medicine)

Adeno-Associated Virus-Mediated Delivery of CRISPR for Cardiac Gene Editing in Mice

Li Xu, Yandi Gao, Yeh Siang Lau, Renzhi Han

¹Department of Surgery, Davis Heart and Lung Research Institute, Biomedical Sciences Graduate Program, Biophysics Graduate Program,Ohio State University Wexner Medical Center

Journal (Genetics)

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins

Harriet Prior*¹, Lauren MacConnachie*¹, Jose L. Martinez, Georgina C.B. Nicholl, Asim A. Beg

¹Department of Pharmacology,University of Michigan

Journal (Genetics)

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

Wing H. Wong*^1,2, R. Spencer Tong*^1,2, Andrew L. Young², Todd E. Druley²

¹Department of Pediatrics, Division of Hematology and Oncology,Washington University School of Medicine, ²Center for Genome Sciences and Systems Biology,Washington University School of Medicine

Journal (Genetics)

Using Mouse Oocytes to Assess Human Gene Function During Meiosis I

Diego Marin*^1,2, Alexandra L. Nguyen*³, Richard T. Scott, Jr.², Karen Schindler

¹IVI-RMA New Jersey, ²Jefferson College of Biomedical Sciences,Thomas Jefferson University, ³Department of Genetics,Rutgers, The State University of New Jersey

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