Poly(A) RNA Purification and Bisulfite Conversion for Next Generation Sequencing (Video) | JoVE

Published: July 07, 2023

doi:

10.3791/200389

¹Institute of Basic Medical Sciences, College of Medicine,National Cheng Kung University, ²Department of Biochemistry and Molecular Biology, College of Medicine,National Cheng Kung University

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Keywords: Poly(A) RNA Purification Bisulfite Conversion Next-generation Sequencing Total RNA Oligo(dT) Beads Lysis Buffer Wash Buffer Tris-HCl RNA Elution Magnetic Separation RNA Sample Preparation RNA Enrichment

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Poly(A) RNA Purification and Bisulfite Conversion for Next Generation Sequencing. J. Vis. Exp. (Pending Publication), e200389, doi:10.3791/200389 (2023).

Poly(A) RNA Purification and Bisulfite Conversion for Next Generation Sequencing (Video) | JoVE

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