Two-Tiered Expansion of Human Mesenchymal Stem Cells from 5-Liter to 15-Liter Bioreactors and Automated Cell Formulation from a 15-Liter Bioreactor (Video) | JoVE

Published: July 07, 2023

doi:

10.3791/200420

Yuyang Chen*¹, Huanye Xu*², Yuanyuan Zhang*², Leilei Guo, Miaomiao Lan, Yanping Yang, Wei Liu, Xiaojun Yan, Yanan Du

¹Department of Biomedical Engineering, School of Medicine,Tsinghua University, ²Beijing CytoNiche Biotechnology Co. Ltd.

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Keywords: Mesenchymal Stem Cells Bioreactor Cell Expansion Automated Cell Formulation Cell Harvest Cell Processing Microcaririer Serum-free Medium Cell Inoculation Medium Supplement Medium Exchange Cell Sampling Cell Harvest Cell Suspension Cell Washing Cell Culture

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Two-Tiered Expansion of Human Mesenchymal Stem Cells from 5-Liter to 15-Liter Bioreactors and Automated Cell Formulation from a 15-Liter Bioreactor. J. Vis. Exp. (Pending Publication), e200420, doi:10.3791/200420 (2023).

Two-Tiered Expansion of Human Mesenchymal Stem Cells from 5-Liter to 15-Liter Bioreactors and Automated Cell Formulation from a 15-Liter Bioreactor (Video) | JoVE

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