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Hepatocellular Carcinoma Tissue Dissociation for Organoid Culture (Video) | JoVE

Published: August 18, 2023

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Cheng-Yang Zhang*^1,2, Xiao-Feng Zhang*³, Jun Yuan*^1,2, Yuan-Feng Gong, Hui Tang, Wan-Yu Guo², Tong-Yan Li², Cai-Wen Li², Yun-Qiang Tang, Ning-Fang Ma², Ming Liu²

¹Affiliated Cancer Hospital and Institute of Guangzhou Medical University, ²Guangzhou Municipal and Guangdong Provincial Key Laboratory of Protein Modification and Degradation, Center for Cancer Research and Translational Medicine, School of Basic Medical Sciences,Guangzhou Medical University, ³Department of Pediatric Surgery, Guangzhou Institute of Pediatrics, Guangzhou Women and Children’s Medical Center,Guangzhou Medical University, ⁴Department of Hepatobiliary Surgery, Affiliated Cancer Hospital,Guangzhou Medical University

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Keywords: Hepatocellular Carcinoma Organoid Culture Basal Medium Digestion Solution Cell Filter Cell Pellet Centrifugation Laminar Flow Hood

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Hepatocellular Carcinoma Tissue Dissociation for Organoid Culture. J. Vis. Exp. (Pending Publication), e200886, doi:10.3791/200886 (2023).

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