Perifusion and Calcium Imaging of Human Pancreatic Tissue Slices for Pancreatic Disease Research

doi:10.3791/201329

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Perifusion and Calcium Imaging of Human Pancreatic Tissue Slices for Pancreatic Disease Research

Published: March 15, 2024

doi:

10.3791/201329

Julia K. Panzer, Pablo A. Garcia, Alberto Pugliese

¹Department of Diabetes Immunology,Arthur Riggs Diabetes and Metabolism Research Institute

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Perifusion Calcium Imaging Pancreatic Disease Research Insulin Secretion Glucagon Detection Agarose Embedding Slice Chamber Baseline Buffer Loading Solutions Chamber Heating Tray Cooling Calcium Dye Confocal Microscopy

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Perifusion and Calcium Imaging of Human Pancreatic Tissue Slices for Pancreatic Disease Research. J. Vis. Exp. (Pending Publication), e201329, doi:10.3791/201329 (2024).

Perifusion and Calcium Imaging of Human Pancreatic Tissue Slices for Pancreatic Disease Research

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