JoVE Journal > Immunology and Infection

Inmunología y contagio

Preparation and Purification of T4 Phage Lysates for Oral Administration in Mice

Published: January 26, 2024

doi:

Nicola Pett, Michael Hunter, Natalia A. Carranza García, Jung Hee Seo, Samuel R. Collins, Forest Rohwer, Lisa C. Osborne, Carolina Tropini^3,4

¹Department of Microbiology and Immunology, Life Sciences Institute,University of British Columbia, ²Department of Biology,San Diego State University, ³School of Biomedical Engineering,University of British Columbia, ⁴Humans and the Microbiome Program,Canadian Institute for Advanced Research

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Keyword Extraction: T4 Phage Escherichia Coli Luria-Bertani (LB) Medium Phage Lysates Oral Administration Centrifugal Filtration Endotoxin Removal Saline Magnesium (SM) Buffer Chloroform Treatment Bacterial Culture Bacterial Debris Removal

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Preparation and Purification of T4 Phage Lysates for Oral Administration in Mice. J. Vis. Exp. (Pending Publication), e201384, doi:10.3791/201384 (2024).

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