Ovarian Fat Pad Transplantation Assay: An In Vivo Technique to Introduce Cells into Ovarian Fat Pad in Mouse Models

Published: April 30, 2023

Abstract

Source: Flesken-Nikitin, A. et al. Transplantation Into the Mouse Ovarian Fat Pad. J. Vis. Exp. (2016)

In this video, we demonstrate an assay for transplantation of experimental cells and tissue fragments into the mouse ovarian fat pad. This method can be used to monitor cell regeneration and tumor transformation via non-invasive techniques.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Ovarian Fat Pad Transplantation NOTE: Disinfect instruments between each animal surgery. Prepare and arrange all equipment in advance. Dorsal transplantations to the right ovarian fat pad are preferential as this avoids the spleen. However, recipients may have transplants …

Divulgaciones

The authors have nothing to disclose.

Materials

28 gauge needle (hypodermic syringe with attached needle)    Kendall 30339 Part Number: 8881500014
Basement membrane matrix
(Geltrex)
Invitrogen  A1413202
Ethanol 200 proof Koptec  V1001  To prepare 70% 
Filter tip 0.1-10/20 µl XL  USA Scientific  1120-3810
Isoflurane, 250 ml  Santa Cruz Animal Health  sc-363629Rx
Ketoprofen Zoetis  4396H
Microscope, stereo Nikon  SMZ800
Nod/SCID/gamma (NSG) mice The Jackson Laboratory 5557 NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ
severe combined
immunodeficiency (SCID/NCr)
mice, BALB/C background)
NCI-Frederick Animal Production Program 01S11

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Ovarian Fat Pad Transplantation Assay: An In Vivo Technique to Introduce Cells into Ovarian Fat Pad in Mouse Models. J. Vis. Exp. (Pending Publication), e20383, doi: (2023).

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