Transverse Aortic Arch Banding in Mouse Model: A Minimally Invasive Technique in Mice for Induction of High Pressure in the Left Ventricle

Abstract

Source: Zaw, A. M. et al. Minimally Invasive Transverse Aortic Constriction in Mice. J. Vis. Exp. (2017)

In this video, we demonstrate an experimental protocol for minimally invasive transverse aortic constriction in mice to examine pressure overload-induced heart failure.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Operation Site Preparation Disinfect the operation site with 75% isopropyl alcohol. Adjust the heating pad temperature to 37 °C to avoid changes in heart and respiratory rates of the subject animal. Use surgical instruments sterilized in a gravity displacement sterilizer at 121 °C fo…

Representative Results

Figure 1: Process of forming the desired needle hooks by bending a 1-in, 30-gauge needle tip. (A) The original 1-in, 30-gauge needle tip. (B) Grasp the needle tip with the needle holder and bend approximately halfway along its length. Bend the needle into a loop, using the needle holder and the hands, as necessary, to form the loop pictured in th…

Materials

1 inch 30-gauge Needles	BD, Franklin Lakes, NJ 07417, USA	305128	Curved as in the procedure and Figure 1
27-gauge Needle	BD, Franklin Lakes, NJ 07417, USA	301629	Make blunt and smooth for spacer
6/0 Dafilon	B. Braun, 34212 Melsungen, Hessen, Germany	C0933066
6/0 Silkam	B. Braun, 34212 Melsungen, Hessen, Germany	C0762067
Sterile gloves	A.R. Medicom, Inc (Asia), Hong Kong
Cotton Applicator	Mannings, Quarry Bay, Hong Kong		Local Shopping Center
Depilatory Cream	Veet (Hong Kong), Kwun Tong, Kowloon, Hong Kong		Local Shopping Center
Lexer-Baby Scissor	FST, British Columbia V7H 0A6, Canada	14078-10
Curved Iris Forcep	FST, British Columbia V7H 0A6, Canada	11065-07
Micro Olsen-Hegar Needle Holder	WPI, Sarasota, FL 34240, USA	501989
Stereo Microscope	WPI, Sarasota, FL 34240, USA	PZMIII-BS
GenTeal Eye Gel	Novartis, East Hanover, NJ 07936, USA		Local Pharmacy
Heating Pad	Kent Scientific Corporation, Torrington, CT 06790, USA	DCT-20
Surgical tape	Laboratory Animal Unit
Ketamine and Xylazine	Laboratory Animal Unit
Betadine	Laboratory Animal Unit
Buprenorphine	Laboratory Animal Unit