Tracer Molecule Delivery in the Cisterna Magna: A Method to Deliver Fluorescent Tracer Molecules via Direct Cannulation of the Cisterna Magna in Pig

Published: April 30, 2023

Abstract

Source: Bèchet, N. B., et al. Direct Cannula Implantation in the Cisterna Magna of Pigs. J. Vis. Exp. (2021).

This video describes the protocol for tracer molecule delivery via direct cannulation of the cisterna magna in a pig brain. This method helps visualize the distribution of cerebrospinal fluid in the glymphatic system.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Cannulation and injection NOTE: This step requires at least two people and is carried out with the animal's head elevated and neck flexed. Begin with an anesthetized pig, pre-operated with surgically accessible cisterna magna. Ensure that one person elevates and flexes the head …

Divulgaciones

The authors have nothing to disclose.

Materials

18G needle Mediq
1ml Syringe FischerSci 15849152
20G cannula Mediq NA
22G cannula Mediq NA
Bovine serum albumin Alexa-Fluor 647 Conjugate ThermoFischer A34785 2 vials (10mg)
CaCl2 Sigmaaldrich C1016
Dental cement Agnthos 7508
Glucose Sigmaaldrich G8270
Insta-Set CA Accelerator BSI-Inc BSI-151
IV line TAP, 3-WAYS with 10cm extension Bbraun NA
KCl Sigmaaldrich P9333
Marker pen NA NA
MgCl2 Sigmaaldrich M8266
MilliQ water NA NA
NaCL Sigmaaldrich S7653
NaH2PO4 Sigmaaldrich S8282
NaHCO3 Sigmaaldrich S5761
Saline Mediq NA
Self-retaining retractors NA NA
Superglue NA NA
Surgical curved scissors NA NA
Surgical towel clamps NA NA

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Tracer Molecule Delivery in the Cisterna Magna: A Method to Deliver Fluorescent Tracer Molecules via Direct Cannulation of the Cisterna Magna in Pig. J. Vis. Exp. (Pending Publication), e20822, doi: (2023).

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