Cellular Impedance-Based Analysis for Monitoring of Cellular Processes in Real-Time

Published: April 30, 2023

Abstract

Source: Labadie, T., et al., Monitoring Influenza Virus Survival Outside the Host Using Real-Time Cell Analysis. J. Vis. Exp. (2021)

In this video, we demonstrate the process of real-time monitoring of cellular events, including the proliferation of adherent cells, via a cellular impedance assay. Here, the adherent cells act as insulators, restricting the current flow between the electrodes and increasing the impedance.

Protocol

NOTE: During all experiments, keep the plates on non-electrostatic surfaces at all times, such as the paper wraps from the packaging. Follow section 1 below to determine the most appropriate concentration of cells to be seeded in the electronic microtiter plate (designed as E-Plate). 1. Determination of appropriate cell quantity for infecting cells Prepare MDCK (Madin-Darby Canine Kidney) cells in 75 cm2 flasks to obtain freshly split cells (approximatel…

Divulgaciones

The authors have nothing to disclose.

Materials

E-Plate 16 (6 plates) ACEA Biosciences, Inc 5469830001 E-plates are available in different packaging
PBS 1X Life technologies (gibco) 14040091
TPCK-Trypsin Worthington LS003740
xCELLigence Real-Time Cell Analysis Instrument S16 ACEA Biosciences, Inc 380601310 The xCELLigence RTCA S16 instruments are available in different formats (16-well, 96-well, single or multi-plate)
 75 cm2 tissue culture flask Falcon 430641U
MEM 1X Life technologies (gibco) 31095029

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Cellular Impedance-Based Analysis for Monitoring of Cellular Processes in Real-Time. J. Vis. Exp. (Pending Publication), e21323, doi: (2023).

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