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人泪液蛋白悬浮捕获样品制备

JoVE Revista
Medicina

This content is Free Access.

JoVE Revista Medicina

Suspension-Trapping Sample Preparation of Human Tear Protein

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人泪液蛋白悬浮捕获样品制备

DOI:

10.3791/200490-v

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03:45 min

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December 01, 2023

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Jimmy Sung-Hei Tse*¹, Ying-Hon Sze*^1,2, Jimmy Ka-Wai Cheung², King-Kit Li, Thomas Chuen Lam^2,3,4

¹Centre for Myopia Research, School of Optometry,The Hong Kong Polytechnic University, ²Centre for Eye and Vision Research (CEVR), ³Research Centre for SHARP Vision (RCSV),The Hong Kong Polytechnic University, ⁴Research Centre for Chinese Medicine Innovation (RCMI),The Hong Kong Polytechnic University

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Suspension-trapping Tear Protein Sample Preparation Dithiothreitol Iodoacetamide Phosphoric Acid Protein Binding Buffer TEAB Digestion Buffer Formic Acid Acetonitrile Gene Ontology Analysis Proteome Data Dependent Acquisition

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