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PCR Amplification and Adaptor Ligation of the Target Sequence for Multi-Gene SNP Detection in Gastric Cancer

JoVE Revista
Medicina

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JoVE Revista Medicina

PCR Amplification and Adaptor Ligation of the Target Sequence for Multi-Gene SNP Detection in Gastric Cancer

PCR Amplification and Adaptor Ligation of the Target Sequence for Multi-Gene SNP Detection in Gastric Cancer

DOI:

10.3791/201593-v

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01:37 min

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May 10, 2024

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Kaihua Huang, Xiao Yan, Zhicheng Huang, Huayuan Liang, Zhiwei Li, Minghao Wang, Yong Wan, Suihai Wang⁴, Liying Zhao

¹Nanfang Hospital, Southern Medical University, ²Research and Development Department,Guangzhou Darui Biotechnology Co., Ltd, ³Key Laboratory of Antibody Engineering of Guangdong Higher Education Institutes, School of Laboratory Medicine and Biotechnology,Southern Medical University, ⁴Provincial Key Laboratory of Immune Regulation and Immunotherapy,Southern Medical University

Tags

Gastric Cancer Single Nucleotide Polymorphisms SNPs 5-fluorouracil 5-FU Pharmacogenetics MTHFR DHFR MTR GGH SLC19A1 Ion Semiconductor Sequencing Next-generation Sequencing

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