Journal
/
Inmunología y contagio
/
Analysis of Pulmonary Dendritic Cell Maturation and Migration during Allergic Airway Inflammation
JoVE Revista
Inmunología y contagio
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Inmunología y contagio
Analysis of Pulmonary Dendritic Cell Maturation and Migration during Allergic Airway Inflammation

Analysis of Pulmonary Dendritic Cell Maturation and Migration during Allergic Airway Inflammation

DOI:
10.3791/4014-v

•

07:52 min

•

July 23, 2012

•

,
1Stem Cell and Cancer Research Institute,McMaster University, Hamilton, 2Physiology and Experimental Medicine Research Program, Hospital for Sick Children,University of Toronto

Capítulos

  • 00:05Título
  • 01:24Sensitizing and Intranasal Challenge of Mice with Ovalbumin
  • 02:39Preparation of Single-cell Suspension from the Lungs and the Draining Lymph Nodes
  • 05:37Results: OVA Sensitization and Challenge Induces an Increase in CD11c+ Cell Trafficking to Murine Mediastinal Lymph Nodes
  • 07:33Conclusion

Summary

Traducción Automática

Traducción Automática

We describe a strategy to monitor maturation and migration of pulmonary dendritic cells in response to ovalbumin in the setting of ovalbumin induced allergic airway inflammation. This strategy can be modified to assess migration of pulmonary dendritic cells in settings of infection.

Tags

Pulmonary Dendritic CellsMaturationMigrationAllergic Airway InflammationAdaptive Immune ResponseAntigen-specific T CellsAntigen StimulationDraining Lymph NodesT Cell ResponseAutoimmune ResponseAllergic Immune ResponseImmune ToleranceLung EnvironmentEnvironmental AntigensAllergyAsthmaAirway DC MaturationAirway DC MigrationSensitizationOvalbumin-induced Allergic Asthma

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

Human T Lymphocyte Isolation, Culture and Analysis of Migration In Vitro

Optimized Protocol for Efficient Transfection of Dendritic Cells without Cell Maturation

Flow Cytometry Analysis of Immune Cells Within Murine Aortas

Monitoring Dendritic Cell Migration using 19F / 1H Magnetic Resonance Imaging

In vivo Imaging Method to Distinguish Acute and Chronic Inflammation

An Optimized Method for Isolating and Expanding Invariant Natural Killer T Cells from Mouse Spleen

Isolation, Characterization and Functional Examination of the Gingival Immune Cell Network

Intra-tracheal Administration of Haemophilus influenzae in Mouse Models to Study Airway Inflammation

A CFSE-based Assay to Study the Migration of Murine Skin Dendritic Cells into Draining Lymph Nodes During Infection with Mycobacterium bovis Bacille Calmette-Guérin

Precision-cut Mouse Lung Slices to Visualize Live Pulmonary Dendritic Cells

Read Article