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Culturing of Human Nasal Epithelial Cells at the Air Liquid Interface
 

Culturing of Human Nasal Epithelial Cells at the Air Liquid Interface

Article DOI: 10.3791/50646-v • 10:38 min • October 8th, 2013
October 8th, 2013 •
1, 1, 1,2, 1,3, 1,2,4
1Center for Environmental Medicine, Asthma, and Lung Biology, The University of North Carolina at Chapel Hill, 2Department of Pediatrics, The University of North Carolina at Chapel Hill, 3Pulmonary Diseases and Critical Care, The University of North Carolina at Chapel Hill, 4Curriculum in Toxicology, The University of North Carolina at Chapel Hill

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Resumen
Automatic Translation

Nasal epithelial cells, obtained through superficial scrape biopsy of human volunteers, are expanded and transferred onto tissue culture inserts. Upon reaching confluency, cells are grown at air liquid interface, yielding cultures of ciliated and non-ciliated cells. Differentiated nasal epithelial cell cultures provide viable experimental models for studying the respiratory mucosa.

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Culturing Human Nasal Epithelial Cells Air-liquid Interface In Vitro Models Respiratory Epithelium Microbial Infections Inhaled Agents Diseased Populations Phenotypes Epithelial Cell Function Tracheobronchial Region Human Lung Tissue Bronchial Brushes Biopsies Nasal Scrape Biopsies Superficial Nasal Scrape Biopsies Respiratory System Air-borne Stressor Coated Tissue Culture Plates Cell Culture Inserts Confluency Physiologically Relevant Conditions ALI Culture Condition Defined Media Differentiated Epithelium
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