Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.
Capítulos
- 00:05Título
- 01:05Vessel Dissection and Imaging
- 03:18Automated Analysis
- 04:36Graphical Output
- 05:28Results: Representative Results from LC_Pro Analysis of Basal and Stimulated Ca2+ Dynamics and Histograms of Parameter Distributions
- 06:23Conclusion
Here a novel region of interest analysis protocol based on sorting best-fit ellipses assigned to regions of positive signal within two-dimensional time lapse image sequences is demonstrated. This algorithm may enable investigators to comprehensively analyze physiological Ca2+ signals with minimal user input and bias.
Tags
Based On The Given TextThe Key Keywords Are:
Automated AnalysisDynamic Ca2+ SignalsImage SequencesIntracellular Ca2+ SignalsFluorescent Ca2+ Indicator DyesMicroscopy TechniquesRegion Of Interest (ROI) DetectionOne-dimensional Line ScanTwo-dimensional Image SequencesEllipsesNoise FilteringCa2+ Signal ParametersAmplitudeDurationSpatial SpreadImageJRStatistical Analysis
