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Performing Subretinal Injections in Rodents to Deliver Retinal Pigment Epithelium Cells in Suspension
 

Performing Subretinal Injections in Rodents to Deliver Retinal Pigment Epithelium Cells in Suspension

Article DOI: 10.3791/52247-v • 06:04 min • January 23rd, 2015
January 23rd, 2015 •
1,2, 1, 1, 1, 2, 1, 1,2
1Department of Cell and Molecular Biology, The Scripps Research Institute, 2Lowy Medical Research Institute

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Here we present a community accepted protocol in multimedia format for subretinally injecting a bolus of RPE cells in rats and mice. This approach can be used for determining rescue potentials, safety profiles, and survival capacities of grafted RPE cells upon implantation in animal models of retinal degeneration.

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Subretinal Injections Rodents Retinal Pigment Epithelium Cells Suspension Light To Electrical Impulses Rod And Cone Photoreceptors Age-related Macular Degeneration AMD Permanent Vision Loss Implantation Of Healthy RPE Cells Pluripotent Stem Cells Safety And Efficacy Animal Models Injecting RPE Cells Creating A Hole In The Eye Sharp Needle Syringe With Blunt Needle Vitreous Integration Of Stem Cell-derived RPE Cells Photoreceptor Degeneration
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