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Comet Assay as an Indirect Measure of Systemic Oxidative Stress
 

Comet Assay as an Indirect Measure of Systemic Oxidative Stress

Article DOI: 10.3791/52763-v • 08:23 min • May 22nd, 2015
May 22nd, 2015 •
1,2, 1,2, 1, 1, 1
1Department of Ophthalmology, University of Basel, 2Department of Biomedicine, University of Basel

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Comet assay measures DNA breaks, induced by different factors. If all factors (except oxidative stress) causing DNA damage are kept constant, the amount of DNA damage is a good indirect parameter of oxidative stress. The goal of this protocol is to use comet assay for indirect measurement of oxidative stress.

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Comet Assay Systemic Oxidative Stress Eukaryotic Organisms Oxygen Molecule Reactive Oxidative Species (ROS) Repair Capacity Oxidative Stress Measurement Cell Gel Electrophoresis DNA Breaks DNA Damage
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